Antiproliferative Effects of Chlorogenic Acid Loaded Chitosan Nanoparticles: An In vitro Approach

In recent years, chlorogenic acid has been reported to have multiple biochemical and pharmacological efficacy including anti-inflammatory and antioxidant properties. The poor solubility and low bioavailability are the major factors that reduce the therapeutic efficacy of the chlorogenic acid. The present study thus synthesized chlorogenic acid loaded chitosan nanoparticles (CACNP) using ion gelation procedure and evaluated its antiproliferative effect against Hep-2 cells under In vitro condition. MTT assay, ROS generation assay, MMP assay and Ao/EtBr assays are utilized to validate its antiproliferative potential in Hep-2 cells. CACNP significantly inhibited Hep-2 cell proliferation. The present study thus reveals the anticancer potential of CACNP, which open avenues for further research to assess its anticancer potential In vivo models.